Journal: Scientific Reports

Article Title: Higher CXCL16 exodomain is associated with aggressive ovarian cancer and promotes the disease by CXCR6 activation and MMP modulation

doi: 10.1038/s41598-019-38766-6

Figure Lengend Snippet: CXCR6 expression in ovarian cancer tissues and cell lines. Ovarian cancer tissues from normal (n = 12), endometrioid (n = 27) and serous (n = 33) were probed with antibody against CXCR6. CXCR6 was developed using DAB (brown). Images (40X) were captured using TissueFAXS cell analysis system from tissuegnostics (Panel A). Immuno-intensity of CXCR6 as shown in box plot was quantified using Histoquest image analysis software (Panel B). Line in the middle represents median value. ★ p < 0.05 when compared to control; ✜ p < 0.05 when compared between the endometrioid and serous papillary groups. CXCR6 protein expression in OvCa and normal epithelial cells was confirmed by FACS analysis. Cells were stained for CXCR6 expression using a purified phycoerythrin-conjugated anti-human CXCR6 (Panel C).

Article Snippet: After blocking, TMA was washed with Tris-buffer, and incubated for 1 hour in humidity chamber at RT with mouse anti-human-CXCR6 monoclonal antibody (1:75 dilution of 1.0 μg/ml stock) (R&D Systems).

Techniques: Expressing, Cell Analysis, Software, Control, Staining, Purification

Journal: Scientific Reports

Article Title: Higher CXCL16 exodomain is associated with aggressive ovarian cancer and promotes the disease by CXCR6 activation and MMP modulation

doi: 10.1038/s41598-019-38766-6

Figure Lengend Snippet: CXCR6-CXCL16 axis promotes ovarian cancer cell migration and invasion. Ovarian cancer cells (OVCAR-3 and SKOV-3) were allowed to migrate (Panel –A,B) or invade (Panel –C,D) in tumor migration and invasion chamber under the chemotactic gradient of CXCL16 (100 ng/ml) with or without anti-CXCR6 antibody (1.0 μg/ml) blocking of CXCR6. Cells that had migrated or invaded through the membrane or matrigel were fixed, stained and counted. Bars represent the mean ± standard error (n = 20) of number of migrated or invaded cells in response to CXCL16. ★★ p < 0.01 represent statistically significant difference between cells migrating/invading towards CXCL16 gradient compared to control and ✜✜ p < 0.01 represent statistically significant difference between cells migrating/invading towards CXCL16 gradient with and without CXCR6 blockade.

Article Snippet: After blocking, TMA was washed with Tris-buffer, and incubated for 1 hour in humidity chamber at RT with mouse anti-human-CXCR6 monoclonal antibody (1:75 dilution of 1.0 μg/ml stock) (R&D Systems).

Techniques: Migration, Blocking Assay, Membrane, Staining, Control

Journal:

Article Title: Single Photon Emission Computed Tomography (SPECT) Experience with ( S )-5-[ 123 I]iodo-3-(2-azetidinylmethoxy)pyridine (5-[ 123 I]IA) in the Living Human Brain of Smokers and Nonsmokers

doi: 10.1002/syn.20611

Figure Lengend Snippet: Characteristics of nuclear neuroimaging of high-affinity α4β2* neuronal nicotinic acetylcholine receptors (nAChRs)

Article Snippet: Brust et al., 2008 , Pigs (mixed German landrace and Pietrain) , 24 , (−)-[ 18 F]NCFHEB, (+)- [ 18 F]NCFHEB, 2- [ 18 F]FA , 420 , 24 CI , HPLC , Kinetic analysis with BioMedical Image Quantification Software PMOD Version 2.75 (PMOD Technologies, Zurich, Switzerland) , (−)-[ 18 F]NCFHEB attains equilibrium before 2-[ 18 F]FA.

Techniques: Clinical Proteomics, Sampling, High Performance Liquid Chromatography, Single Photon Emission Computed Tomography, Radioactivity, Imaging, Gas Chromatography, Software, Binding Assay, Protein Binding